Purification and characterization of polyethylene glycol dehydrogenase involved in the bacterial metabolism of polyethylene glycol
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چکیده
منابع مشابه
Purification and characterization of polyethylene glycol dehydrogenase involved in the bacterial metabolism of polyethylene glycol.
Polyethylene glycol (PEG) dehydrogenase in crude extracts of a PEG 20,000-utilizing mixed culture was purified 24 times by precipitation with ammonium sulfate, solubilization with laurylbetaine, and chromatography with diethylamino-ethyl-cellulose, hydroxylapatite, and Sephadex G-200. The purified enzyme was confirmed to be homogeneous by sodium dodecyl sulfate-polyacrylamide gel electrophoresi...
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Apostolos Mantides MD, Head, Department of Gastroenterology, Athens Naval and Veterans Hospital, Tel & fax: ++3
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1980
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.40.4.701-705.1980